Glycan-related products

●Glycan related products

Our products are listed below.
Details by clicking the product name.

●Our expertise

Technical experts will provide service for R＆D on glycan chemistry.
Our technical expertise will meet your request. Please feel free to consult with us.

Chemical modification
of glyco-protein

Details follow.
Can be open by PDF mode.

Conjugation reaction
of glycan with amine

Details follow.
Can be open by PDF mode.

Oxazoline forming
reaction of glycan

Details follow.
(We will introduce "CDMBI")

Addition reaction of
α2,3-sialic acid

We wait for your requests.
Please use the attached
form for questions.

●Product Info

Sialylglycopeptide（SGP；α2,6-SGP）

■Product No.:171801
■Product Name:Sialylglycopeptide(SGP)
■Formula，Molecular Weight:
C₁₁₂H₁₈₉N₁₅O₇₀, 2,865.76
■Assay:min. 95% (HPLC)
■Package:10mg,100mg,1g

α2,3-SGP

■Product No.:171803
■Product Name:α2,3-SGP
■Formula，Molecular Weight:
C₁₁₂H₁₈₉N₁₅O₇₀, 2,865.76
■Assay:min. 95% (HPLC)
■Package:1mg, 10mg

Asialo-SGP

■Product No.:171804
■Product Name:Asialo-SGP
■Formula，Molecular Weight:
C₉₀H₁₅₅N₁₃O₅₄, 2,283.25
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Agalacto-SGP

■Product No.:171816
■Product Name:Agalacto-SGP
■Formula，Molecular Weight:
C₇₈H₁₃₅N₁₃O₄₄, 1,958.97
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Sialylglycan（SG；α2,6-SG）

■Product No.:171808
■Product Name:Sialylglycan
■Formula，Molecular Weight:
C₇₆H₁₂₅N₅O₅₇, 2,020.80
■Assay:min. 95% (HPLC)
■Package:10mg,100mg,1g,10g

Asialoglycan（G2-Glycan）

■Product No.:171817
■Product Name:Asialoglycan, G2-Glycan
■Formula，Molecular Weight:
C₅₄H₉₁N₃O₄₁, 1,438.30
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Agalactoglycan（G0-Glycan）

■Product No.:171818
■Product Name:Agalactoglycan, G0-Glycan
■Formula，Molecular Weight:
C₄₂H₇₁N₃O₃₁, 1,114.01
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Sialylglycan-Oxazoline（SG-Oxazoline）

■Product No.:171810
■Product Name:Sialylglycan-Oxazoline
■Formula，Molecular Weight:
C₇₆H₁₂₃N₅O₅₆, 2,002.79
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Asialoglycan-Oxazoline（G2-Oxazoline）

■Product No.:171814
■Product Name:Asialoglycan-Oxazoline,
G2-Oxazoline
■Formula，Molecular Weight:
C₅₄H₈₉N₃O₄₀, 1,420.28
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Agalactoglycan-Oxazoline（G0-Oxazoline）

■Product No.:171815
■Product Name:Agalactoglycan-Oxazoline,
G0-Oxazoline
■Formula，Molecular Weight:
C₄₂H₆₉N₃O₃₀, 1,096.00
■Assay:min. 95% (HPLC)
■Package:1mg,10mg

Endo-CC

■Product No.:171831
■Product Name:Endo-CC
■Package:300munits
■Source:Endo-CC recombinant from Coprinopsis cinerea is expressed in E.coli as a fusion to His tag.
■Unit Definition:One unit is defined as the amount of enzyme that produce 1μmol SG from SGP per minute at 37℃, pH 8.0.

Endo-CC N180H

■Product No.:171832
■Product Name:
Endo-CC N180H
■Package:300munits
■Source:Endo-CC N180H recombinant from Coprinopsis cinerea is expressed in E.coli as a fusion to His tag.
■Unit Definition:One unit is defined as the amount of enzyme that produce 1μmol SG-GlcNAc-pNP from pNP-GlcNAc per minute at 30˚C, pH7.5.

Endo-β-N-acetylglucosaminidase (ENGase) catalyzes hydrolysis of N-linked oligosaccharides.
In addition, some ENGases have transglycosylation activity, and can transfer sugar chains to sugar or glycoconjugate as acceptor.

Endo-CC is an ENGase from the basidiomycete fungus Coprinopsis cinerea, and catalyzes hydrolysis of N-linked oligosaccharides, especially high-mannose type and biantennary complex type.
Endo-CC N180H is Endo-CC mutant, and can transfer sugar chains for example SG-Oxazoline (product number:171810) as donor to GlcNAc as acceptor.

Reaction example (Can be open by PDF mode.)

Reference:

1) Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K:
Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea.
PloS one 10, e0132859 (2015)
2) Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K:
Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline.
Biotechnol. Lett. 39, 157-162 (2017)
3) Manabe S, Yamaguchi Y, Abe J, Matsumoto K, Ito Y:
Acceptor range of endo-β-N-acetylglucosaminidase mutant endo-CC N180H:from monosaccharide to antibody.
R Soc Open Sci, 5 (5) , 171521 2018 16 eCollection May (2018)

CDMBI

■Product No.:171811
■Product Name:CDMBI
2-Chloro-1,3-dimethyl-1H-benzimidazol-3-ium chloride
■Formula，Molecular Weight:
C₉H₁₀ClN₂Cl, 217.10
■Assay:min. 95% (NMR)
■Package:1g,5g,25g

●Publications

1) Ashida H, Fujimoto T, Kurihara S, Nakamura M, Komeno M, Huang Y, Katayama T, Kinoshita T, Takegawa K:

1,6-α-L-Fucosidases from Bifidobacterium longum subsp. infantis ATCC 15697 involved in the degradation of core-fucosylated N-glycan.
Journal of Applied Glycoscience 67, 23-29 (2020)
DOI:10.5458/jag.jag.JAG-2019_0016

2) Seki H, Huang Y, Arakawa T, Yamada C, Kinoshita T, Iwamoto S, Higuchi Y, Takegawa K, Fushinobu S:

Structural basis for the specific cleavage of core-fucosylated N-glycans by endo-β-N-acetylglucosaminidase from the fungus Cordyceps militaris.
J Biol Chem. 294, 17143-17154 (2019)
PMID:31548313 DOI:10.1074/jbc.RA119.010842

3) Manabe S, Yamaguchi Y, Matsumoto K, Fuchigami H, Kawase T, Hirose K, Mitani A, Sumiyoshi W, Kinoshita T, Abe J, Yasunaga M, Matsumura Y, Ito Y:

Characterization of antibody products obtained through enzymatic and nonenzymatic glycosylation reactions with a glycan oxazoline and preparation of a homogeneous antibody-drug conjugate via Fc N-glycan.
Bioconjug Chem.30, 1343-1355 (2019)
PMID:30938513 DOI:10.1021/acs.bioconjchem.9b00132

4) Huang Y, Higuchi Y, Kinoshita T, Mitani A, Eshima Y, Takegawa K:

Characterization of novel endo-β-N-acetylglucosaminidases from Sphingobacterium species, Beauveria bassiana and Cordyceps militaris that specifically hydrolyze fucose-containing oligosaccharides and human IgG.
Sci. Rep. 8, 246 (2018)
PMID:29321565 DOI:10.1038/s41598-017-17467-y.

5) Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K:

Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline.
Biotechnol. Lett.39, 157-162 (2017)
PMID:27714557 DOI:10.1007/s10529-016-2230-0

6) Suda M, Sumiyoshi W, Kinoshita T, Ohno S:

Reaction of sugar oxazolines with primary amines
Tetrahedron Letters 57, 5446–5448 (2016)
DOI:10.1016/j.tetlet.2016.10.074

7) Eshima Y, Higuchi Y, Kinoshita T, Nakakita S, Takegawa K:

Transglycosylation activity of glycosynthase mutants of endo-β-N-acetylglucosaminidase from Coprinopsis cinerea.
PLoS ONE 10, e0132859 (2015)
PMID:26197478 DOI:10.1371/journal.pone.0132859